| Field | Specification |
|---|---|
| Target | |
| Alternative names | B-cell lymphoma/leukemia 11B; BCL-11B; B-cell CLL/lymphoma 11B; COUP-TF-interacting protein 2; Radiation-induced tumor suppressor gene 1 protein; hRit1; BCL11B; CTIP2; RIT1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BCL11B recombinant protein (Position: Q10-A628). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets B-cell lymphoma/leukemia 11B (gene BCL11B) in human and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 95.5 kDa, and the supplier reports an observed band at about 116 kDa.
BCL11B is a zinc finger transcription factor that is essential for T-cell commitment and development in the thymus, and it helps maintain mature T-cell identity. It is also needed for development of neurons in the cortex and striatum and for skin and tooth formation, and it is studied in T-cell leukemia and immunodeficiency.
| Target | B-cell lymphoma/leukemia 11B (gene BCL11B; UniProt Q9C0K0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 95.5 kDa |
| Observed MW | 116 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Rat) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MOLT-4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human bladder urothelial carcinoma with squamous differentiation tissue, human breast cancer tissue, human chronic tonsillitis tissue, human lung adenocarcinoma tissue, human lymph node metastasis carcinoma tissue, human placenta tissue, human thyroid papillary carcinoma tissue, human ovary serous carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): C6 cells.
Immunogen
Recombinant human BCL11B fragment (Gln10–Ala628), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places B-cell lymphoma/leukemia 11B in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenjie Wang et al. (2026) Engineered brain organoids on a 3D scaffold reveal synaptic-related toxicity of benzophenone-3 associated with Wnt/β-catenin dysregulation. Environmental Technology & Innovation. 10.1016/j.eti.2026.105011
