| Field | Specification |
|---|---|
| Target | |
| Alternative names | Broad substrate specificity ATP-binding cassette transporter ABCG2; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; Urate exporter; CD antigen CD338; ABCG2; ABCP; BCRP; BCRP1; MXR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BCRP/ABCG2 recombinant protein (Position: M1-R378). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects broad substrate specificity ATP-binding cassette transporter ABCG2 in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 72.3 kDa, and the supplier reports an observed band at about 65–80 kDa.
BCRP (ABCG2) is an ATP-dependent efflux transporter with broad substrate range, including many anticancer drugs, dietary toxins, urate and porphyrins. It is found in the intestine, placenta, blood-brain barrier and stem cells, and it contributes to multidrug resistance and to gout risk.
| Target | Broad substrate specificity ATP-binding cassette transporter ABCG2 (gene ABCG2; UniProt Q9UNQ0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 72.3 kDa |
| Observed MW | 65–80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human, Mouse) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A549 cells, human MDA-MB-453 cells, human HepG2 cells, human Caco-2 cells, human HCC1937 cells, human PC-3 cells, human MDA-MB-231 cells, human HeLa cells, human MCF-7 cells, human T47D cells, rat liver tissue, rat kidney tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue, mouse liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human BCRP/ABCG2 fragment (Met1–Arg378), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places broad substrate specificity ATP-binding cassette transporter ABCG2 in the cell membrane, apical cell membrane and mitochondrion membrane. Tissue expression noted by UniProt (human): Highly expressed in placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenhua Guo et al. (2024) Structural characterization and anti-hyperuricemic effect of a mannogalactan from Armillariella tabescens mycelium. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.137152
Jinghua Duan et al. (2012) Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2012.01.020
Yang Zhou et al. (2024) Herb–drug interactions: Quantitative analysis of levofloxacin absorption and transporter expression in the rat intestine following combined treatment with Persicaria capitata (Buch.-Ham. ex D. Don) H. Gross. Journal of Pharmaceutical and Biomedical Analysis. 10.1016/j.jpba.2024.116156
Ting-Zhun Zhu et al. (2014) β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells. International Journal of Oncology. 10.3892/ijo.2014.2448
Bin Yu et al. (2013) The mechanism of the opening of the blood–brain barrier by borneol: A pharmacodynamics and pharmacokinetics combination study. Journal of Ethnopharmacology. 10.1016/j.jep.2013.10.028
Bin Qiao et al. (2011) Disclosure of a stem cell phenotype in an oral squamous cell carcinoma cell line induced by BMP-4 via an epithelial-mesenchymal transition. Oncology Reports. 10.3892/or.2011.1299
