| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Beta-2-microglobulin; B2m |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human BCRP/ABCG2 recombinant protein (Position: M1-R378). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-BCRP/ABCG2 Antibody Picoband® is an antibody for ABCG2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ABCG2 (beta-2 microglobulin); UniProt: Q9UNQ0
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 65-80 kDa, calculated 22875 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-BCRP/ABCG2 Antibody Picoband® catalog # A00457-2.
Biological background
Biological context: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Expression and localization notes: cellular localization: Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized., tissue context: Detected in lung, spleen, uterus, vagina and epididymis. ..
Common research applications
- Western blotting (WB): Compare ABCG2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ABCG2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify ABCG2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: ABCG2 (Atp-binding cassette, subfamily g, member 2) also known as ABCP, BCRP or MRX, is a protein that in humans is encoded by the ABCG2 gene. It is mapped on 4q22.1. The ABCG2 gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes. The ABCG2 protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with ABCG2 expression that was stimulated by compounds known to be transported by this protein. ABCG2 is likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels. Abcg2 transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food. ABCG2 is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Tissue details: Detected in lung, spleen, uterus, vagina and epididymis. .
- Research category: Immunoglobulins,Immunology
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
