| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Beclin-1;Coiled-coil myosin-like BCL2-interacting protein;Protein GT197;BECN1;GT197; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Beclin 1, identical to the related rat and mouse sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Beclin 1/BECN1 Antibody Picoband® is an antibody targeting BECN1. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Rat,Mouse,Human; observed MW: 46 kDa; calculated MW: 51896 MW.
Boster Bio Anti-Beclin 1/BECN1 Antibody catalog # PA1847. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: BECN1 — Beclin-1
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Rat,Mouse,Human
- Molecular weight guidance: Observed: 46 kDa; Calculated: 51896 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Plays a central role in autophagy (PubMed:23184933). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3- C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense. .
Scientific background (datasheet): Bellini-1 (beclin 1, autophagy related) is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene (Atg) 6. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. Human beclin encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread beclin expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed beclin immunoreactivity in many neurons throughout these regions, as well as in some glial cells. Beclin protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that beclin reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.
Cellular localization (datasheet): Cytoplasm . Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Endosome . Cytoplasmic vesicle, autophagosome . Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity). .
Tissue details (datasheet): Ubiquitous.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cancer Susceptibility,Cell Death,Epigenetics and Nuclear Signaling,Interspecies Interaction,Metabolism,Metabolism Processes,Microbiology,Oncoproteins/Suppressors,Pathways and Processes,Signal Transduction,Transcription,Tumor Suppressors.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
