| Field | Specification |
|---|---|
| Target | |
| Alternative names | Beclin-1;Coiled-coil myosin-like BCL2-interacting protein;Protein GT197;BECN1;GT197; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Beclin 1, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets beclin-1 (gene BECN1) in rat, mouse and human samples and is validated for Western blot. The predicted molecular weight is 51.9 kDa, and the supplier reports an observed band at about 46 kDa.
Beclin-1 (BECN1) is a core subunit of the class III phosphatidylinositol 3-kinase (VPS34) complex, which generates phosphatidylinositol 3-phosphate to start autophagosome formation. It also functions in endocytic trafficking, and its activity is controlled by binding partners such as Bcl-2. Beclin-1 is a widely used marker in autophagy research.
| Target | Beclin-1 (gene BECN1; UniProt Q14457, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 51.9 kDa |
| Observed MW | 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): HeLa cells, SW620 cells, PANC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the middle region of human beclin 1. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt places beclin-1 in the cytoplasm, endosome membrane, endoplasmic reticulum membrane and mitochondrion membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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