| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bestrophin-1; TU15B; Vitelliform macular dystrophy protein 2; BEST1; VMD2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Bestrophin, which shares 62.5% amino acid (aa) sequence identity with mouse Bestrophin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of BEST1 (Keratin, type I cytoskeletal 14) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Bestrophin/BEST1 Antibody Picoband® catalog # A01434-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human Bestrophin, which shares 62.5% amino acid (aa) sequence identity with mouse Bestrophin.
- Molecular weight context: reported MW: 68 kDa; calculated MW: 68 kDa
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Keratin, type I cytoskeletal 14; bestrophin 1. Bestrophin-1 (Best1) is a protein that, in humans, is encoded by the BEST1 gene. This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms. Functional note: Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate. Reported localization: Cell membrane. Expression/tissue context: Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.
Research relevance and current trends
- Cell Adhesion Proteins: Researchers commonly examine how BEST1 (Keratin, type I cytoskeletal 14) relates to this theme using model systems and orthogonal readouts.
- ECM Proteins: Researchers commonly examine how BEST1 (Keratin, type I cytoskeletal 14) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how BEST1 (Keratin, type I cytoskeletal 14) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative BEST1 (Keratin, type I cytoskeletal 14) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
