| Field | Specification |
|---|---|
| Target | |
| Alternative names | Beta-2 adrenergic receptor;Beta-2 adrenoreceptor;Beta-2 adrenoceptor;ADRB2;ADRB2R, B2AR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ADRB2, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes beta-2 adrenergic receptor (gene ADRB2) in human and rat samples and is validated for Western blot. The predicted molecular weight is 46.5 kDa, and the supplier reports an observed band at about 47 kDa.
Beta-2 adrenergic receptor (ADRB2) is a G protein-coupled receptor for epinephrine that relaxes smooth muscle in the airways and blood vessels. It is the target of asthma bronchodilators and has been a key model receptor for GPCR structure and signaling.
| Target | Beta-2 adrenergic receptor (gene ADRB2; UniProt P07550, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 46.5 kDa |
| Observed MW | 47 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat, Human) |
|---|
Samples with a confirmed band (WB): rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the middle region of human ADRB2. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places beta-2 adrenergic receptor in the cell membrane and Golgi apparatus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lei Yang et al. (2021) Epoxyeicosatrienoic acids prevent cardiomyocytes against sepsis by A2AR-induced activation of PI3K and PPARγ. Prostaglandins & Other Lipid Mediators. 10.1016/j.prostaglandins.2021.106595