| Field | Specification |
|---|---|
| Target | |
| Alternative names | Catenin beta-1;Beta-catenin;CTNNB1;CTNNB;OK/SW-cl.35, PRO2286; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human beta Catenin, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects catenin beta-1 (gene CTNNB1) in rat, mouse and human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 85.5 kDa, and the supplier reports an observed band at about 95 kDa.
β-Catenin (CTNNB1) has two roles: it is a structural part of cadherin-based adherens junctions and the central effector of canonical Wnt signaling. Without Wnt, a destruction complex of APC, AXIN, CK1α and GSK3B targets it for proteasomal degradation; with Wnt, it builds up in the nucleus and activates TCF/LEF target genes. It is studied in development, stem cells and cancer.
| Target | Catenin beta-1 (gene CTNNB1; UniProt P35222, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Mouse, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 85.5 kDa |
| Observed MW | 95 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat, Human) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): MM453 cells, MCF-7 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (IHC): rat intestine tissue.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Synthetic peptide from the C-terminal region of human beta-catenin. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with rat, mouse and human. UniProt places catenin beta-1 in the cytoplasm, nucleus, cell junction and cell membrane. Tissue expression noted by UniProt (human): Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Shenglan et al. (2025) Effects of solamargine in hepatic metastasis of colorectal cancer: induction of ferroptosis and elimination of cancer stem cells. Chinese Medicine. 10.1186/s13020-025-01171-5
Zhong Gang et al. (2018) Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway. Cellular Physiology and Biochemistry. 10.1159/000495926
Hai-yan Piao et al. (2021) LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer. Journal of Cancer. 10.7150/jca.49138
Hong-ping Hao et al. (2015) LiCl inhibits PRRSV infection by enhancing Wnt/β-catenin pathway and suppressing inflammatory responses. Antiviral Research. 10.1016/j.antiviral.2015.02.010
Jiatao Wu et al. (2024) Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer. Scientific Reports. 10.1038/s41598-024-63055-2
Shenghui Liu (2024) AHR regulates liver enlargement and regeneration through the YAP signaling pathway. Heliyon. 10.1016/j.heliyon.2024.e37265
Jun-Yan Cai et al. (2018) Histone deacetylase HDAC4 promotes the proliferation and invasion of glioma cells. International Journal of Oncology. 10.3892/ijo.2018.4564
Kuangyu Jin et al. (2026) STEAP1 Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt/β‐Catenin Signalling and EMT. Cancer Medicine. 10.1002/cam4.71862
Ma Junjun et al. (2025) Saracatinib Delays the Progression of Renal Interstitial Fibrosis by Inhibiting the Wnt/β-Catenin Pathway. Kidney360. 10.34067/KID.0000000972
Jianfeng Mu et al. (2018) Retracted: Curcumin derivative L6H4 inhibits proliferation and invasion of gastric cancer cell line BGC-823. Journal of Cellular Biochemistry. 10.1002/jcb.27542
