| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tubulin beta-3 chain;Tubulin beta-4 chain;Tubulin beta-III;TUBB3;TUBB4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human beta III Tubulin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-TUBB3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone CC-20; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody catalog # M01857-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TUBB3 (Tubulin beta-3 chain).
- Antibody format: Monoclonal; clone CC-20; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
TUBB3 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. . Reported cellular localization context: Cytoplasm, cytoskeleton. Tissue expression notes (as provided): Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. .
Research relevance and current trends
- Research context keywords from the source record include: Cell Adhesion Proteins,Cell Type Markers,Cytoskeleton,Cytoskeleton/ECM,Microtubules,Neuroscience,Signal Transduction,Tubulin.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate TUBB3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect TUBB3 expression by Western blot in cell or tissue lysates, Detect TUBB3 in FFPE tissue sections by immunohistochemistry, Localize TUBB3 by immunofluorescence/immunocytochemistry in cultured cells, Quantify TUBB3-positive cells by flow cytometry in single-cell suspensions, Enrich TUBB3 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 50 kDa; calculated MW: 50433 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 50 kDa
- Cellular localization (provided): Cytoplasm, cytoskeleton.
- Tissue details (provided): Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
