| Field | Specification |
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| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
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| Reconstitution | |
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| UniProt # |
Overview
Anti-Beta Tubulin Antibody Picoband®(monoclonal, 5E4) is an antibody for TUBB detection raised in Mouse (Monoclonal, clone Clone: 5E4, Mouse IgG1), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: TUBB; UniProt: P07437
- Antibody format: Mouse, Monoclonal, clone Clone: 5E4, Mouse IgG1
- Molecular weight: 55 kDa, calculated 25035 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Beta Tubulin Antibody Picoband® (monoclonal, 5E4) catalog # M01857-3.
Biological background
Biological context: Involved in redox regulation of the cell. Can reduce H (2)O (2) and short chain organic,fatty acid and phospholipid hydroperoxides.May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Expression and localization notes: cellular localization: Cytoplasm . Lysosome . Cytoplasmic vesicle . Also found in lung secretory organelles., tissue context: Widely expressed in fetal and adult tissues..
Common research applications
- Western blotting (WB): Compare TUBB levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of TUBB in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify TUBB-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytoplasm . Lysosome . Cytoplasmic vesicle . Also found in lung secretory organelles.
- Tissue details: Widely expressed in fetal and adult tissues.
- Research category: Actin Binding Proteins,Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Organelle Proteins,Protein Trafficking,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
