| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tubulin beta-3 chain;Tubulin beta-4 chain;Tubulin beta-III;TUBB3;TUBB4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human beta Tubulin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated is an antibody targeting TUBB3. Common applications include WB. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: DF-20; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 68 kDa; calculated MW: 50433 MW.
Boster Bio Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated catalog # H01857. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TUBB3 — Tubulin beta-3 chain
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: DF-20; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 68 kDa; Calculated: 50433 MW
Biological background
Protein function (datasheet): Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. .
Cellular localization (datasheet): Cytoplasm, cytoskeleton.
Tissue details (datasheet): Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. .
Research relevance and current trends
- Commonly studied in contexts related to Cell Adhesion Proteins,Cell Type Markers,Cytoskeleton,Cytoskeleton/ECM,Microtubules,Neuroscience,Signal Transduction,Tubulin.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
