| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tubulin beta chain; Tubulin beta-5 chain; TUBB; TUBB5; OK/SW-cl.56 |
| Cellular Localization | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) is an antibody for TUBB detection raised in Mouse (Monoclonal, clone Clone: 2E11, Mouse IgG2a), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: TUBB (tubulin beta class I); UniProt: P07437
- Antibody format: Mouse, Monoclonal, clone Clone: 2E11, Mouse IgG2a
- Molecular weight: 55 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Beta Tubulin TUBB Antibody Picoband® (monoclonal, 2E11) catalog # M05613-4.
Biological background
Biological context: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Expression and localization notes: cellular localization: cytoskeleton, tissue context: Ubiquitously expressed with highest levels in spleen, thymus and immature brain..
Common research applications
- Western blotting (WB): Compare TUBB levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of TUBB in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify TUBB-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: cytoskeleton
- Tissue details: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
- Research category: Cell Adhesion Proteins,Epigenetics and Nuclear Signaling,Integrins,Mediator Complex,Neuroscience,Receptors,Transcription
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
