| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tubulin beta-1 chain;TUBB1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Tubulin from rat brain. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) is an antibody targeting TUBB1. Common applications include WB, IHC, ICC, IF. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: TUB 2.1; isotype: Mouse IgG1; reactivity: Human,Mouse,Rat; observed MW: 55 kDa; calculated MW: 50327 MW.
Boster Bio Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) catalog # MA1112. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: TUBB1 — Tubulin beta-1 chain
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: TUB 2.1; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 55 kDa; Calculated: 50327 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). .
Scientific background (datasheet): Tubulin beta (TUBB) was derived from its corresponding mRNA with a molecular weight of about 55,000. TUBB gene contains 4 exons and located in the HLA class I region at 6p21.3. Paclitaxel resistance in non-small-cell lung cancer associated with beta-tubulin gene mutations.
Cellular localization (datasheet): Cytoplasm, cytoskeleton .
Tissue details (datasheet): Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. .
Sequence similarities (datasheet): Belongs to the glycoprotein hormones subunit beta family.
Research relevance and current trends
- Commonly studied in contexts related to Cytoskeleton,Cytoskeleton/ECM,Microtubules,Signal Transduction,Subcellular Markers,Tags & Cell Markers,Tubulin.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
