| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 14-3-3 protein beta/alpha;Protein 1054;Protein kinase C inhibitor protein 1;KCIP-1;14-3-3 protein beta/alpha, N-terminally processed;YWHAB; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Bmf |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-BMF antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22B83; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, Flow (as provided in the source record). Boster Bio Anti-Bmf Rabbit Monoclonal Antibody catalog # M02432-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: BMF (14-3-3 protein beta/alpha).
- Antibody format: Monoclonal; clone 22B83; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
BMF (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activatation of MAP kinases via AKAP13. . Reported cellular localization context: Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue expression notes (as provided): Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. .
Research relevance and current trends
- Research context keywords from the source record include: Adapters,Cell Type Marker,Cytoplasmic,Neural Signal Transduction,Neurology Process,Neuron Marker,Neuroscience,Signal Transduction,Signaling Pathways,Stem Cells,Synapse Marker.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate BMF antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect BMF expression by Western blot in cell or tissue lysates, Quantify BMF-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 24 kDa; calculated MW: 28082 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 24 kDa
- Cellular localization (provided): Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Tissue details (provided): Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
