| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl-2-modifying factor; BMF |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Bmf |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22B83 is a rabbit monoclonal antibody that recognizes bcl-2-modifying factor (gene BMF) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 20.5 kDa, and the supplier reports an observed band at about 24 kDa.
BMF (Bcl-2-modifying factor) is a BH3-only pro-apoptotic Bcl-2 family protein. In healthy cells it is held on the actin cytoskeleton through dynein light chain 2, and it is released upon loss of cell attachment or other stresses to bind anti-apoptotic Bcl-2 proteins and promote apoptosis, including anoikis.
| Target | Bcl-2-modifying factor (gene BMF; UniProt Q96LC9, human) |
|---|---|
| Clone | 22B83 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.5 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human bmf.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Isoform 1 is mainly expressed in B-lymphoid cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
