| Field | Specification |
|---|---|
| Target | |
| Alternative names | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP3; NIP3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BNIP3, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (gene BNIP3) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 22.0 kDa, and the supplier reports an observed band at about 28–30 kDa.
BNIP3 is a BH3-containing protein of the mitochondrial outer membrane that is strongly induced by hypoxia through HIF-1. It promotes mitochondrial autophagy (mitophagy) by binding LC3 family proteins and can also contribute to cell death, and it is studied in hypoxia, cardiac injury and cancer.
| Target | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (gene BNIP3; UniProt Q12983, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 22.0 kDa |
| Observed MW | 28–30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HepG2 cells, human A431 cells, human U251 cells, rat liver tissue, rat RH35 cells, mouse liver tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human BNIP3. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 in the mitochondrion and mitochondrion outer membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xue Li et al. (2025) Gymconopin C exhibits anti-non-small cell lung cancer effect by regulating miR-6777-5p/ADRB2 pathway to promote mitophagy. Journal of Advanced Research. 10.1016/j.jare.2025.12.023
Yan Du et al. (2017) MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling. Oncotarget. 10.18632/oncotarget.18604
Jing-jing Xing et al. (2019) Ginsenoside Rb3 provides protective effects against cisplatin-induced nephrotoxicity via regulation of AMPK-/mTOR-mediated autophagy and inhibition of apoptosis in vitro and in vivo. Cell Proliferation. 10.1111/cpr.12627
Hongjuan Ning et al. (2020) β-arrestin1 inhibits hypoxic injury-induced autophagy in human pulmonary artery endothelial cells via the Akt/mTOR signaling pathway. International Journal of Biochemistry & Cell Biology. 10.1016/j.biocel.2020.105791
Haihua Zhang et al. (2026) Gentiana szechenyii Kanitz Compounds Regulate Lipopolysaccharide-Induced Inflammation and Mitochondrial Autophagy Via the Toll-Like Receptor 4-Nuclear Factor Kappa B Pathway. Chemistry & Biodiversity. 10.1002/cbdv.202503838
