| Field | Specification |
|---|---|
| Target | |
| Alternative names | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3;BNIP3;NIP3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BNIP3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAEI-2 is a rabbit monoclonal antibody that recognizes BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (gene BNIP3) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 22.0 kDa; the supplier reports observed bands at 30, 50 kDa.
BNIP3 is a BH3-containing protein of the mitochondrial outer membrane that is strongly induced by hypoxia through HIF-1. It promotes mitochondrial autophagy (mitophagy) by binding LC3 family proteins and can also contribute to cell death, and it is studied in hypoxia, cardiac injury and cancer.
| Target | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (gene BNIP3; UniProt Q12983, human) |
|---|---|
| Clone | AAEI-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.0 kDa |
| Observed MW | 30, 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human pancreatic cancer tissue, human placenta tissue, human thyroid cancer tissue, human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human BNIP3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 in the mitochondrion and mitochondrion outer membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sandra Franco-Iborra et al. (2020) Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease. Autophagy. 10.1080/15548627.2020.1728096
Huan Deng et al. (2025) Chondroitin sulfate A-selenium nanoparticles protect chondrocytes from T-2 toxin-induced oxidative stress and mitochondrial dysfunction through activating autophagy by the SIRT1-AMPK-FOXO3 pathway. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2025.118797
Yong-Cai Zhao et al. (2021) Hypoxic training upregulates mitochondrial turnover and angiogenesis of skeletal muscle in mice. Life Sciences. 10.1016/j.lfs.2021.119340
