| Field | Specification |
|---|---|
| Target | |
| Alternative names | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like;Adenovirus E1B19K-binding protein B5;BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A;NIP3-like protein X;NIP3L;BNIP3L;BNIP3A, BNIP3H, NIX; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BNIP3L |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABIB-2 is a rabbit monoclonal antibody that recognizes BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (gene BNIP3L) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 23.9 kDa, and the supplier reports an observed band at about 35 kDa.
BNIP3L (also called NIX) is a mitochondrial outer membrane protein related to BNIP3. It acts as a mitophagy receptor that binds LC3/GABARAP proteins and is required for mitochondrial clearance during red blood cell maturation. It can also promote apoptosis under some conditions.
| Target | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (gene BNIP3L; UniProt O60238, human) |
|---|---|
| Clone | ABIB-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.9 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human BNIP3L.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like in the nucleus envelope, endoplasmic reticulum, mitochondrion outer membrane and membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sandra Franco-Iborra et al. (2020) Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease. Autophagy. 10.1080/15548627.2020.1728096
Wenxiu Liu et al. (2025) Aconiti Lateralis Radix Praeparata ameliorates heart failure via PI3K/AKT/Bnip3 pathway. Frontiers in Pharmacology. 10.3389/fphar.2025.1526653
Hongjuan Ning et al. (2020) β-arrestin1 inhibits hypoxic injury-induced autophagy in human pulmonary artery endothelial cells via the Akt/mTOR signaling pathway. International Journal of Biochemistry & Cell Biology. 10.1016/j.biocel.2020.105791
Jia-Xiang Zhang et al. (2023) Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells. World Journal of Gastrointestinal Oncology. 10.4251/wjgo.v15.i3.464
