| Field | Specification |
|---|---|
| Target | |
| Alternative names | Breast cancer type 1 susceptibility protein;6.3.2.-;RING finger protein 53;BRCA1;RNF53; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BRCA1 recombinant protein (Position: Q1467-Y1863). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes breast cancer type 1 susceptibility protein (gene BRCA1) in human samples and is validated for Western blot, flow cytometry and ELISA.
BRCA1 is a tumor suppressor that forms an E3 ubiquitin ligase with BARD1 and acts in several nuclear complexes that sense and repair DNA double-strand breaks, especially by homologous recombination. It also supports cell cycle checkpoints and genome stability, and inherited BRCA1 mutations greatly increase the risk of breast and ovarian cancer.
| Target | Breast cancer type 1 susceptibility protein (gene BRCA1; UniProt P38398, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human BRCA1 fragment (Gln1467–Tyr1863), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places breast cancer type 1 susceptibility protein in the nucleus, chromosome and cytoplasm. Tissue expression noted by UniProt (human): Isoform 1 and isoform 3 are widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
