| Field | Specification |
|---|---|
| Target | |
| Alternative names | Breast cancer metastasis-suppressor 1;BRMS1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BRMS1 recombinant protein (Position: D52-D244). Human BRMS1 shares 93.6% and 96% amino acid (aa) sequence identity with mouse and rat BRMS1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects breast cancer metastasis-suppressor 1 (gene BRMS1) in hamster samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 28.5 kDa, and the supplier reports an observed band at about 29 kDa.
BRMS1 was identified as a breast cancer metastasis suppressor that inhibits metastasis without blocking primary tumor growth. It is a component of SIN3–HDAC corepressor complexes and represses gene transcription, including dampening NF-κB activity by promoting deacetylation of RELA.
| Target | Breast cancer metastasis-suppressor 1 (gene BRMS1; UniProt Q9HCU9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Hamster |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 28.5 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human MCF-7 cells, human 293T cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Recombinant human BRMS1 fragment (Asp52–Asp244), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.6% and 96%, respectively.
Reactivity Notes
The supplier lists reactivity with hamster. UniProt places breast cancer metastasis-suppressor 1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expression levels are higher in term placentas than in early placentas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
