| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitotic checkpoint serine/threonine-protein kinase BUB1; mBUB1; BUB1A; Bub1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Bub1 recombinant protein (Position: D40-K257). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone Clone: 5E12 is a rabbit polyclonal antibody that recognizes mitotic checkpoint serine/threonine-protein kinase BUB1 in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 119.6 kDa, and the supplier reports an observed band at about 122 kDa.
BUB1 is a mitotic serine/threonine kinase that binds kinetochores and is needed for the spindle assembly checkpoint and correct chromosome alignment. It phosphorylates histone H2A at Thr120, which recruits shugoshin to protect centromeric cohesion and helps localize the chromosomal passenger complex.
| Target | Mitotic checkpoint serine/threonine-protein kinase BUB1 (gene Bub1; UniProt O08901, mouse) |
|---|---|
| Clone | Clone: 5E12 |
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 119.6 kDa |
| Observed MW | 122 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse testis tissue, rat heart tissue, rat PC-12 wholel cells, rat RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells.
Immunogen
Recombinant mouse Bub1 fragment (Asp40–Lys257), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places mitotic checkpoint serine/threonine-protein kinase BUB1 in the nucleus and chromosome. Tissue expression noted by UniProt (mouse): Highly expressed in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
