| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mast/stem cell growth factor receptor Kit; SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; KIT; SCFR |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse c-Kit/Kit, which shares 88.6% and 94.3% amino acid (aa) sequence identity with human and rat c-Kit/Kit, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mast/stem cell growth factor receptor Kit in human samples and is validated for Western blot and IHC. The predicted molecular weight is 109.3 kDa, and the supplier reports an observed band at about 145 kDa.
KIT (CD117) is a receptor tyrosine kinase activated by stem cell factor (SCF). It supports the survival and proliferation of hematopoietic stem cells, mast cells, melanocytes and germ cells, and activating KIT mutations drive gastrointestinal stromal tumors and some mast cell and blood cancers.
| Target | Mast/stem cell growth factor receptor Kit (gene Kit; UniProt P05532, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 109.3 kDa |
| Observed MW | 145 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human 293T cells, human K562 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue, human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of mouse c-Kit/Kit. Sequence identity with the human and rat orthologs: 88.6% and 94.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (mouse): Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yudong Wei et al. (2017) Double sex and mab-3 related transcription factor 1 regulates differentiation and proliferation in dairy goat male germline stem cells. Journal of Cellular Physiology. 10.1002/jcp.26129
Jian Yang et al. (2015) Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.12548
Jingjin Liu et al. (2013) Wnt1 Inhibits Hydrogen Peroxide-Induced Apoptosis in Mouse Cardiac Stem Cells. PLoS One. 10.1371/journal.pone.0058883
Lingling Wang et al. (2017) Identification and Distribution of the Interstitial Cells of Cajal in the Abomasum of Goats. Cell Transplantation. 10.1177/0963689717722561
Wen-Ting Bin et al. (2012) Embryonic hepatocyte transplantation for hepatic cirrhosis: Efficacy and mechanism of action. World Journal of Gastroenterology. 10.3748/wjg.v18.i4.309
Hongyun Liu et al. (2005) Stimulating effects of androgen on proliferation of cultured ovarian germ cells through androgenic and estrogenic actions in embryonic chickens. Domestic Animal Endocrinology. 10.1016/j.domaniend.2005.02.018
Xinyan Tang et al. (2006) Activation of protein kinases A and C promoted proliferation of chicken primordial germ cells. Animal Reproduction Science. 10.1016/j.anireprosci.2006.09.020
Lei Zhou et al. (2018) Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature. Therapeutics and Clinical Risk Management. 10.2147/TCRM.S167248
Yuling Mi et al. (2004) Effects of follicle-stimulating hormone and androgen on proliferation of cultured testicular germ cells of embryonic chickens. General and Comparative Endocrinology. 10.1016/j.ygcen.2004.06.012
Chutian Ge et al. (2013) Ginsenosides promote proliferation of chicken primordial germ cells via PKC-involved activation of NF-κB. Cell Biology International. 10.1016/j.cellbi.2007.05.001
