| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mast/stem cell growth factor receptor Kit;SCFR;2.7.10.1;Piebald trait protein;PBT;Proto-oncogene c-Kit;Tyrosine-protein kinase Kit;p145 c-kit;v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog;CD117;KIT;SCFR; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human c-Kit |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-c-Kit Rabbit Monoclonal Antibody is an antibody targeting KIT. Common applications include WB, IHC. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: CGF-11; isotype: Rabbit IgG; reactivity: Human; observed MW: 17 kDa; calculated MW: 109865 MW.
Boster Bio Anti-c-Kit Rabbit Monoclonal Antibody catalog # M01335-1. Tested in WB, IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Target: KIT — Mast/stem cell growth factor receptor Kit
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: CGF-11; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 17 kDa; Calculated: 109865 MW
Biological background
Protein function (datasheet): Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. .
Cellular localization (datasheet): Isoform 1: Cell membrane; Single-pass type I membrane protein.
Tissue details (datasheet): Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. .
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cell Type Markers,Cytokines,Developmental Biology,Germ Cell Markers,Germline Stem Cells,Hematopoietic Progenitors,Immunology,Myeloid,Neuroscience,Neurotransmission,Oncoproteins,Oncoproteins/Suppressors,Organogenesis,Protein Phosphorylation,Receptor Tyrosine Kinases,Receptors / Channels,Reproduction,Signal Transduction,Stem Cells,Surface Molecules,Tags & Cell Markers,Tumor Associated,Tyrosine Kinases.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
