| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor Maf;Proto-oncogene c-Maf;V-maf musculoaponeurotic fibrosarcoma oncogene homolog;MAF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human c-Maf |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23M12 is a rabbit monoclonal antibody that recognizes transcription factor Maf in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 38.5 kDa, and the supplier reports an observed band at about 44 kDa.
MAF (c-Maf) is a large Maf-family basic leucine zipper transcription factor. It regulates crystallin genes during lens fiber development and controls key immune genes, notably IL-4 in T helper 2 cells and IL-10 in several immune cell types, and it is also studied in multiple myeloma, where MAF translocations occur.
| Target | Transcription factor Maf (gene MAF; UniProt O75444, human) |
|---|---|
| Clone | 23M12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 38.5 kDa |
| Observed MW | 44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HUVEC cells, NIH/3T3 cells, PC12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human c-Maf.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor Maf in the nucleus. Tissue expression noted by UniProt (human): Expressed in endothelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
