| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcriptional activator Myb; Proto-oncogene c-Myb; MYB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human c-Myb |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20M27 is a rabbit monoclonal antibody that recognizes transcriptional activator Myb in human samples and is validated for Western blot and IHC. The predicted molecular weight is 72.3 kDa, and the supplier reports an observed band at about 78 kDa.
c-Myb (MYB) is a transcription factor that binds specific DNA motifs and controls genes for proliferation and differentiation. It is essential for hematopoietic progenitor cells and is also expressed in colon epithelium and neural progenitors. MYB is deregulated in leukemias and some solid tumors.
| Target | Transcriptional activator Myb (gene MYB; UniProt P10242, human) |
|---|---|
| Clone | 20M27 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 72.3 kDa |
| Observed MW | 78 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): MOLT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human c-Myb.
Reactivity Notes
The supplier lists reactivity with human. UniProt places transcriptional activator Myb in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
