| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Myc proto-oncogene protein;Proto-oncogene c-Myc;Transcription factor p64;Myc; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-c-Myc Antibody (Monoclonal, 9E10) is an antibody targeting MYC. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: 9E10; isotype: Mouse IgG1; reactivity: Human; observed MW: 49 kDa; calculated MW: 48898 MW.
Boster Bio Anti-c-Myc Antibody (Monoclonal, 9E10) catalog # MA1028. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: MYC — Myc proto-oncogene protein
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: 9E10; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 49 kDa; Calculated: 48898 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes. .
Scientific background (datasheet): C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
Cellular localization (datasheet): Nucleus, nucleoplasm . Nucleus, nucleolus .
Tissue details (datasheet): Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .
Sequence similarities (datasheet): Contains 1 bHLH (basic helix-loop-helix) domain.
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cell Cycle,Cell Differentiation,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Nuclear,Oncoproteins,Oncoproteins/Suppressors,Signaling Pathways,Stem Cells,TGF Beta,Transcription,Transcription Factors,Tumor Biomarkers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
