| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myc proto-oncogene protein;Class E basic helix-loop-helix protein 39;bHLHe39;Proto-oncogene c-Myc;Transcription factor p64;MYC;BHLHE39; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human c-Myc recombinant protein (Position: E257-A439). Human c-Myc shares 91% amino acid (aa) sequences identity with both mouse and rat c-Myc. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects myc proto-oncogene protein in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 48.8 kDa, and the supplier reports an observed band at about 60–65 kDa.
c-Myc (MYC) is a bHLH-leucine zipper transcription factor that pairs with MAX, binds E-box sequences and broadly amplifies the transcription of genes for growth, ribosome biogenesis, metabolism and proliferation. It also supports stem cell self-renewal and reprogramming. MYC is one of the most frequently deregulated oncogenes in human cancer.
| Target | Myc proto-oncogene protein (gene MYC; UniProt P01106, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 48.8 kDa |
| Observed MW | 60–65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human K562 cells, rat C6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Recombinant human c-Myc fragment (Glu257–Ala439), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places myc proto-oncogene protein in the nucleus, cytoplasm and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shi Jingjing et al. (2023) Feiyiliu Mixture sensitizes EGFRDel19/T790M/C797S mutant non-small cell lung cancer to osimertinib by attenuating the PRC1/Wnt/EGFR pathway. Frontiers in Pharmacology. 10.3389/fphar.2023.1093017
Huicui Yang et al. (2011) RPB5-mediating Protein Is Required for the Proliferation of Hepatocellular Carcinoma Cells *. Journal of Biological Chemistry. 10.1074/jbc.M110.136929
Jiatao Wu et al. (2024) Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer. Scientific Reports. 10.1038/s41598-024-63055-2
Chunyan Zhang et al. (2017) The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation. PLoS One. 10.1371/journal.pone.0180607
Xiao-Bo Long et al. (2009) Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer. Oncology Reports. 10.3892/or_00000554
Zhang Chunyan et al. (2017) The novel protein C3orf43 accelerates hepatocyte proliferation. Cellular & Molecular Biology Letters. 10.1186/s11658-017-0051-3
Dong-Hui Xu et al. (2008) Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells. World Journal of Gastroenterology. 10.3748/wjg.14.5008