Anti-c-Myc Antibody Picoband®

SKU:BHA21000004
Research Validated
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Overview
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Anti-MYC antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunofluorescence and Western blot. Reactivity: Human, Mouse, Rat (species coverage varies by application). Recognizes myc proto-oncogene protein.
Target MYC
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) IF, ICC, WB
Options selector
Catalog no. Size Conjugation
PB9092 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: IF,ICC,WB
      Application details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

      Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
      Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MYC
Alternative names Myc proto-oncogene protein;Class E basic helix-loop-helix protein 39;bHLHe39;Proto-oncogene c-Myc;Transcription factor p64;MYC;BHLHE39;
UniProt # P01106
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunofluorescence
  • Western Blot
Immunogen E.coli-derived human c-Myc recombinant protein (Position: E257-A439). Human c-Myc shares 91% amino acid (aa) sequences identity with both mouse and rat c-Myc.
Molecular weight 48.8 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Nucleus, nucleoplasm . Nucleus, nucleolus .
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) PB9092
Main SKU BHA21000004
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody detects myc proto-oncogene protein in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 48.8 kDa, and the supplier reports an observed band at about 60–65 kDa.

c-Myc (MYC) is a bHLH-leucine zipper transcription factor that pairs with MAX, binds E-box sequences and broadly amplifies the transcription of genes for growth, ribosome biogenesis, metabolism and proliferation. It also supports stem cell self-renewal and reprogramming. MYC is one of the most frequently deregulated oncogenes in human cancer.

Target Myc proto-oncogene protein (gene MYC; UniProt P01106, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4
Calculated MW 48.8 kDa
Observed MW 60–65 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.1–0.5 µg/mL (Human, Mouse, Rat)
Immunocytochemistry / Immunofluorescence 5 µg/mL (Human)

Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human K562 cells, rat C6 cells, mouse NIH/3T3 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.

Samples with confirmed staining (ICC/IF): HeLa cells.

Immunogen

Recombinant human c-Myc fragment (Glu257–Ala439), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91%.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places myc proto-oncogene protein in the nucleus, cytoplasm and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

For research use only; not for diagnostic or therapeutic procedures. Handle with standard laboratory precautions.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Human, Mouse, Rat); Immunocytochemistry / Immunofluorescence 5 µg/mL (Human). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 48.8 kDa; observed bands at about 60 and 65 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Recombinant human c-Myc fragment (Glu257–Ala439), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91%.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Shi Jingjing et al. (2023) Feiyiliu Mixture sensitizes EGFRDel19/T790M/C797S mutant non-small cell lung cancer to osimertinib by attenuating the PRC1/Wnt/EGFR pathway. Frontiers in Pharmacology. 10.3389/fphar.2023.1093017

Huicui Yang et al. (2011) RPB5-mediating Protein Is Required for the Proliferation of Hepatocellular Carcinoma Cells *. Journal of Biological Chemistry. 10.1074/jbc.M110.136929

Jiatao Wu et al. (2024) Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer. Scientific Reports. 10.1038/s41598-024-63055-2

Chunyan Zhang et al. (2017) The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation. PLoS One. 10.1371/journal.pone.0180607

Xiao-Bo Long et al. (2009) Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer. Oncology Reports. 10.3892/or_00000554

Zhang Chunyan et al. (2017) The novel protein C3orf43 accelerates hepatocyte proliferation. Cellular & Molecular Biology Letters. 10.1186/s11658-017-0051-3

Dong-Hui Xu et al. (2008) Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells. World Journal of Gastroenterology. 10.3748/wjg.14.5008

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