| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiopoietin-like protein 8 ;Betatrophin ;Lipasin ;Refeeding-induced fat and liver protein ;ANGPTL8 ;C19orf80, RIFL ;UNQ599/PRO1185 ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human C19orf80 recombinant protein (Position: A22-A198). Human C19orf80 shares 74% amino acid (aa) sequence identity with mouse C19orf80. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes angiopoietin-like protein 8 (gene ANGPTL8) in human samples and is validated for Western blot. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 22 kDa.
ANGPTL8 (also called betatrophin) is a liver and fat-derived hormone that regulates plasma triglycerides. Induced by feeding, it works with ANGPTL3 to inhibit lipoprotein lipase in muscle, directing fatty acids toward storage in adipose tissue after a meal.
| Target | Angiopoietin-like protein 8 (gene ANGPTL8; UniProt Q6UXH0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 22.1 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human C19orf80 fragment (Ala22–Ala198), expressed in E. coli. Sequence identity with the mouse ortholog: 74%.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes angiopoietin-like protein 8 as a secreted protein. Tissue expression noted by UniProt (human): Predominantly expressed in liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
