| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3; CPAMD1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human C3 recombinant protein (Position: Q1277-E1538). Human C3 shares 73.7 % and 76.3% amino acid (aa) sequence identity with mouse and rat C3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects complement C3 in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 187.1 kDa; the supplier reports observed bands at 128 kDa, 187 kDa.
Complement C3 is the central protein of the complement system, where the classical, lectin and alternative pathways converge. Cleavage by C3 convertases produces C3b, which coats pathogens and cells for phagocytosis and forms C5 convertases, and C3a, an anaphylatoxin that promotes inflammation. It is one of the most studied markers of complement activation in immunity and inflammatory disease.
| Target | Complement C3 (gene C3; UniProt P01024, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 187.1 kDa |
| Observed MW | 128 kDa, 187 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, rat plasma, mouse liver tissue, mouse plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human C3 fragment (Gln1277–Glu1538), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes complement C3 as secreted and also located in the cell surface. Tissue expression noted by UniProt (human): Plasma... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuanyuan Wang et al. (2024) Antimicrobial peptide-modified pH-sensitive micelles against pneumonia caused by methicillin-resistant Staphylococcus aureus. Journal of Drug Delivery Science and Technology. 10.1016/j.jddst.2024.105875
Ying Xue et al. (2024) DJ-1 regulates astrocyte activation through miR-155/SHP-1 signaling in cerebral ischemia/reperfusion injury. Journal of Neurochemistry. 10.1111/jnc.16230
Jianchao Mao et al. (2024) Modulation of GPER1 alleviates early brain injury via inhibition of A1 reactive astrocytes activation after intracerebral hemorrhage in mice. Heliyon. 10.1016/j.heliyon.2024.e26909
Yinghui Song et al. (2026) Localization of the Complement C1q-Binding Site on Echinococcus multilocularis Calreticulin Identified by Peptide Mapping. Tropical Medicine and Infectious Disease. 10.3390/tropicalmed11060146
