| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Complement C3;C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1;Complement C3 beta chain;C3-beta-c;C3bc;Complement C3 alpha chain;C3a anaphylatoxin;Acylation stimulating protein;ASP;C3adesArg;Complement C3b alpha' chain;Complement C3c alpha' chain fragment 1;Complement C3dg fragment;Complement C3g fragment;Complement C3d fragment;Complement C3f fragment;Complement C3c alpha' chain fragment 2;C3;CPAMD1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human C3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-C3/Complement C3 Rabbit Monoclonal Antibody is an antibody targeting C3. Common applications include WB, ICC, IF, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOHE-3; isotype: Rabbit IgG; reactivity: Human; observed MW: 30 kDa; calculated MW: 187148 MW.
Boster Bio Anti-C3/Complement C3 Rabbit Monoclonal Antibody catalog # M00168. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: C3 — Complement C3
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOHE-3; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 30 kDa; Calculated: 187148 MW
Biological background
Protein function (datasheet): C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Cellular localization (datasheet): Secreted.
Tissue details (datasheet): Plasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. .
Research relevance and current trends
- Commonly studied in contexts related to Apoptosis,Cancer,Cancer Susceptibility,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,DNA/RNA,DNA Damage & Repair,DNA Damage Response,Epigenetics and Nuclear Signaling,Intracellular,Oncoproteins/Suppressors,p53 Pathway,Transcription,Tumor Suppressors.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
