| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanine nucleotide exchange C9orf72; C9orf72 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human C9ORF72, which shares 96.4% amino acid (aa) sequence identity with both mouse and rat C9ORF72. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects guanine nucleotide exchange factor C9orf72 in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 54.3 kDa, and the supplier reports an observed band at about 65 kDa.
C9orf72 is a guanine nucleotide exchange factor for Rab GTPases that works in a complex with SMCR8 and WDR41 to regulate autophagy and lysosomal function. A hexanucleotide repeat expansion in the C9ORF72 gene is the most common genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia, which makes the protein a major focus of neurodegeneration research.
| Target | Guanine nucleotide exchange factor C9orf72 (gene C9orf72; UniProt Q96LT7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 54.3 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| Immunohistochemistry | Not specified by the supplier |
Samples with a confirmed band (WB): human placenta tissue, human U2OS cells, human PC-3 cells, human K562 cells, human HeLa cells, human A431 cells, human A549 cells, rat brain tissue, rat liver tissue, rat C6 cells, mouse liver tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Synthetic peptide from the N-terminal region of human C9ORF72. Sequence identity with the mouse and rat orthologs: 96.4%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places guanine nucleotide exchange factor C9orf72 in the cytoplasm, nucleus, endosome and lysosome. Tissue expression noted by UniProt (human): Both isoforms are widely expressed, including kidney, lung, liver, heart, testis and several brain regions, such as cerebellum. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
