| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carbonic anhydrase 9;4.2.1.1;Carbonate dehydratase IX;Carbonic anhydrase IX;CA-IX;CAIX;Membrane antigen MN;P54/58N;Renal cell carcinoma-associated antigen G250;RCC-associated antigen G250;pMW1;CA9;G250, MN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CA9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EFE-3 is a rabbit monoclonal antibody that recognizes carbonic anhydrase 9 (gene CA9) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 49.7 kDa, and the supplier reports an observed band at about 55 kDa.
Carbonic anhydrase 9 (CA9, also called CAIX) is a transmembrane enzyme that converts CO2 and water into bicarbonate and protons at the cell surface. Its expression is strongly induced by hypoxia through HIF-1, and it is a well-known marker of tumor hypoxia and clear cell renal cell carcinoma.
| Target | Carbonic anhydrase 9 (gene CA9; UniProt Q16790, human) |
|---|---|
| Clone | EFE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 49.7 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human stomach.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kindey cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human CA9.
Reactivity Notes
The supplier lists reactivity with human. UniProt places carbonic anhydrase 9 in the nucleus, cell membrane and cell projection. Tissue expression noted by UniProt (human): Expressed primarily in carcinoma cells lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaoli Zhang et al. (2019) Sohlh2 alleviates malignancy of EOC cells under hypoxia via inhibiting the HIF1α/CA9 signaling pathway. Biological Chemistry. 10.1515/hsz-2019-0119
