| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Calbindin;Calbindin D28;D-28K;Vitamin D-dependent calcium-binding protein, avian-type;CALB1;CAB27; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Calbindin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CALB1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AAAA-3; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF (as provided in the source record). Boster Bio Anti-Calbindin CALB1 Rabbit Monoclonal Antibody catalog # M03047. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CALB1 (Calbindin).
- Antibody format: Monoclonal; clone AAAA-3; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CALB1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Buffers cytosolic calcium. May stimulate a membrane Ca (2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Reported cellular localization context: Cytoplasm . Cell membrane ; Peripheral membrane protein . Tissue expression notes (as provided): Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. .
Research relevance and current trends
- Research context keywords from the source record include: Calcium Binding Proteins,Calcium Signaling,Cell Type Marker,Neuron Marker,Neuroscience,Neurotransmission.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate CALB1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CALB1 expression by Western blot in cell or tissue lysates, Detect CALB1 in FFPE tissue sections by immunohistochemistry, Localize CALB1 by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 65 kDa; calculated MW: 30025 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 65 kDa
- Cellular localization (provided): Cytoplasm . Cell membrane ; Peripheral membrane protein .
- Tissue details (provided): Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
