| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Calcineurin subunit B type 1;Protein phosphatase 2B regulatory subunit 1;Protein phosphatase 3 regulatory subunit B alpha isoform 1;Ppp3r1;Cna2, Cnb; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Bovine brain calcineurin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1) is an antibody targeting PPP3R1. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: CN-A1; isotype: Mouse IgG1; reactivity: Bovine,Human,Mouse,Rat; observed MW: 61 kDa; calculated MW: 19300 MW.
Boster Bio Anti-Calcineurin Alpha Ppp3r1 Antibody (Monoclonal, CN-A1) catalog # MA1008. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.
Key elements and design rationale
- Target: PPP3R1 — Calcineurin subunit B type 1
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: CN-A1; Isotype: Mouse IgG1
- Species reactivity: Bovine,Human,Mouse,Rat
- Molecular weight guidance: Observed: 61 kDa; Calculated: 19300 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Scientific background (datasheet): Calcineurin, the Ca (2+)/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in all cells from yeast to mammals. Calcineurin A alpha (PPP3CA),is located on human chromosomes 4, Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit. Calcineurin regulates bone formation by the osteoblast.
Cellular localization (datasheet): Cytoplasm, cytosol . Cell membrane ; Lipid-anchor . Cell membrane, sarcolemma. Translocates from the cytosol to the sarcolemma in a CIB1- dependent manner during cardiomyocytes hypertrophy. .
Tissue details (datasheet): Isoform 2 is testis specific. .
Sequence similarities (datasheet): Belongs to the calcineurin regulatory subunit family.
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,B Cells,Calcium Signaling,Calmodulin Pathway,Immunology,Non-CD,Protein Phosphorylation,Ser/Thr Phosphatases,Signal Transduction,Signaling Pathway.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
