| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Calmodulin;CaM;Calm1;Calm, Cam, Cam1;Calm2;Cam2, Camb;Calm3;Cam3, Camc; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Calmodulin Calm1 Antibody (Monoclonal, 6D4) is an antibody targeting CALM1; CALM2; CALM3. Common applications include WB. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: 6D4; isotype: Mouse IgG1; reactivity: Chicken,Rat; observed MW: 61 kDa; calculated MW: 16838 MW.
Boster Bio Anti-Calmodulin Calm1 Antibody (Monoclonal, 6D4) catalog # MA1010. Tested in WB applications. This antibody reacts with Chicken, Rat.
Key elements and design rationale
- Target: CALM1; CALM2; CALM3 — Calmodulin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: 6D4; Isotype: Mouse IgG1
- Species reactivity: Chicken,Rat
- Molecular weight guidance: Observed: 61 kDa; Calculated: 16838 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca (2+). Among the enzymes to be stimulated by the calmodulin-Ca (2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (By similarity). .
Scientific background (datasheet): Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters. Three calmodulin genes (CALM1, CALM2, and CALM3) map to chromosomes 14q24-q31, 2p21.1-p21.3, and 19q13.2-q13.3, respectively.
Cellular localization (datasheet): Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. .
Tissue details (datasheet): High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Sequence similarities (datasheet): Belongs to the calmodulin family.
Research relevance and current trends
- Commonly studied in contexts related to Apoptosis,Atherosclerosis,Calcium Signaling,Calmodulin Pathway,Cancer,Cancer Metabolism,Carbohydrate Metabolism,Cardiovascular,Heart Disease,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism Of Carbohydrates,Pathways and Processes,Signal Transduction,Signaling Pathway.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
