| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Calretinin;CR;29 kDa calbindin;CALB2;CAB29; |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminal of human Calretinin, different from the related mouse and rat sequences by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CALB2 (Calretinin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Calretinin/CALB2 Antibody Picoband® catalog # PB9248. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminal of human Calretinin, different from the related mouse and rat sequences by one amino acid.
- Molecular weight context: reported MW: 38 kDa; calculated MW: 31540 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Calretinin; Calretinin. Calretinin, also known as 29 kDa calbindin, is a vitamin D-dependent calcium-binding protein involved in calcium signaling. In humans, the calretinin protein is encoded by the CALB2 gene. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Calretinin is mapped to 16q22.2. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Functional note: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Expression/tissue context: Brain.
Research relevance and current trends
- Calcium Binding Proteins: Researchers commonly examine how CALB2 (Calretinin) relates to this theme using model systems and orthogonal readouts.
- Calcium Signaling: Researchers commonly examine how CALB2 (Calretinin) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how CALB2 (Calretinin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CALB2 (Calretinin) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CALB2 (Calretinin) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the calbindin family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
