Anti-Carbonic anhydrase 2 Rabbit Monoclonal Antibody

Overview

This product is an anti-CA2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 17C55; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-Carbonic anhydrase 2 Rabbit Monoclonal Antibody catalog # M00143-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: CA2 (B-cell lymphoma 6 protein).
• Antibody format: Monoclonal; clone 17C55; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

CA2 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding ly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6- binding site) or inly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, inly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4 (+) T-cells (T (FH) cells), promotes the expression of T (FH)-related genes but inhibits the differentiation of T (H)1, T (H)2 and T (H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53- dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH- dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation. . Reported cellular localization context: Nucleus . Tissue expression notes (as provided): Expressed in germinal center T- and B-cells and in primary immature dendritic cells. .

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cancer Susceptibility,Domain Families,Epigenetics and Nuclear Signaling,Oncoproteins,Oncoproteins/Suppressors,Proto-Oncogenes,Transcription,Transcription Factors,Zinc Finger.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.

Workflow ideas (metafield): Validate CA2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CA2 expression by Western blot in cell or tissue lysates, Detect CA2 in FFPE tissue sections by immunohistochemistry

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 95 kDa; calculated MW: 78846 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 95 kDa
• Cellular localization (provided): Nucleus .
• Tissue details (provided): Expressed in germinal center T- and B-cells and in primary immature dendritic cells. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.