| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II; Cyanamide hydratase CA2; CA2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Carbonic anhydrase 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17C55 is a rabbit monoclonal antibody that recognizes carbonic anhydrase 2 (gene CA2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 29.2 kDa, and the supplier reports an observed band at about 29 kDa.
Carbonic anhydrase 2 (CA2) is a fast, widely expressed cytosolic enzyme that converts CO2 and water into bicarbonate and protons. It is essential for acid secretion by osteoclasts during bone resorption and contributes to fluid secretion in the eye and kidney, making it a classic target in studies of pH regulation, osteopetrosis and glaucoma.
| Target | Carbonic anhydrase 2 (gene CA2; UniProt P00918, human) |
|---|---|
| Clone | 17C55 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.2 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human carbonic anhydrase 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places carbonic anhydrase 2 in the cytoplasm and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
