| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carbonic anhydrase 4;4.2.1.1;Carbonate dehydratase IV;Carbonic anhydrase IV;CA-IV;CA4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CA IV recombinant protein (Position: A19-S284). Human CA IV shares 55.5% and 58.9% amino acid (aa) sequence identity with mouse and rat CA IV, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets carbonic anhydrase 4 (gene CA4) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 35.0 kDa, and the supplier reports an observed band at about 35 kDa.
Carbonic anhydrase 4 (CA4) is a GPI-anchored zinc enzyme on the outer surface of endothelial and epithelial cells. It converts CO2 into bicarbonate and protons to maintain extracellular and intracellular pH and supports bicarbonate reabsorption in the kidney. It is studied in renal acid–base handling, retinal biology and capillary endothelium.
| Target | Carbonic anhydrase 4 (gene CA4; UniProt P22748, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 35.0 kDa |
| Observed MW | 35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|
Samples with a confirmed band (WB): rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human CA IV fragment (Ala19–Ser284), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 55.5% and 58.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places carbonic anhydrase 4 in the cell membrane. Tissue expression noted by UniProt (human): Expressed in the endothelium of the choriocapillaris in eyes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
