| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Carboxypeptidase A1; CPA1; CPA |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Carboxypeptidase A, which shares 84.8% and 87% amino acid (aa) sequence identity with mouse and rat Carboxypeptidase A, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CPA1 (Long-chain-fatty-acid--CoA ligase 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Carboxypeptidase A/CPA1 Antibody Picoband® catalog # A05985-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human Carboxypeptidase A, which shares 84.8% and 87% amino acid (aa) sequence identity with mouse and rat Carboxypeptidase A, respectively.
- Molecular weight context: reported MW: 47 kDa; calculated MW: 80420 MW
- Reactivity: Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Long-chain-fatty-acid--CoA ligase 3; carboxypeptidase A1. Carboxypeptidase A1 is an enzyme that in humans is encoded by the CPA1 gene. This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer. Functional note: Carboxypeptidase that catalyzes the release of a C- terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro. Reported localization: Secreted. Expression/tissue context: Expressed in testis, colon, thymus and in breast cancer.
Research relevance and current trends
- Cancer: Researchers commonly examine how CPA1 (Long-chain-fatty-acid--CoA ligase 3) relates to this theme using model systems and orthogonal readouts.
- Metabolic Signaling Pathways: Researchers commonly examine how CPA1 (Long-chain-fatty-acid--CoA ligase 3) relates to this theme using model systems and orthogonal readouts.
- Metabolism: Researchers commonly examine how CPA1 (Long-chain-fatty-acid--CoA ligase 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CPA1 (Long-chain-fatty-acid--CoA ligase 3) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
