| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Signal transducer and activator of transcription 4;STAT4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human CARD11 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CARD11 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23C93; isotype IgG; reactivity: Human,Mouse. Reported application contexts include WB, ICC, IF (as provided in the source record). Boster Bio Anti-CARD11 Rabbit Monoclonal Antibody catalog # M00740. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: CARD11 (Signal transducer and activator of transcription 4).
- Antibody format: Monoclonal; clone 23C93; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CARD11 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling. Reported cellular localization context: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. Tissue expression notes (as provided): Ubiquitous. Expressed at high levels in testis.
Research relevance and current trends
- Research context keywords from the source record include: DNA/RNA,Epigenetics and Nuclear Signaling,RNA Processing.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate CARD11 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CARD11 expression by Western blot in cell or tissue lysates, Localize CARD11 by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 133 kDa; calculated MW: 85941 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 133 kDa
- Cellular localization (provided): Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
- Tissue details (provided): Ubiquitous. Expressed at high levels in testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
