| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NLR family CARD domain-containing protein 4;CARD, LRR, and NACHT-containing protein;Clan protein;Caspase recruitment domain-containing protein 12;Ice protease-activating factor;Ipaf;NLRC4;CARD12, CLAN, CLAN1, IPAF;UNQ6189/PRO20215; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of NLRC4 (NLR family CARD domain-containing protein 4) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CARD12/NLRC4 Antibody Picoband® catalog # PB9673. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human CARD12, different from the related mouse sequence by five amino acids, and from the related rat sequence by seven amino acids.
- Molecular weight context: reported MW: 116 kDa; calculated MW: 116159 MW
- Reactivity: Human,Mouse,Rat
- Applications: IF, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
NLR family CARD domain-containing protein 4; NLR family CARD domain-containing protein 4. NLR family CARD domain-containing protein 4, also known as CARD12, is a protein that in humans is encoded by the NLRC4 gene. It is mapped to 2p22.3. This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants. Functional note: Key component of inflammasomes that inly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. . Reported localization: Cytoplasm . Cytoplasm, cytosol . Cytoplasmic filaments. Expression/tissue context: Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.
Research relevance and current trends
- Apoptosis: Researchers commonly examine how NLRC4 (NLR family CARD domain-containing protein 4) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how NLRC4 (NLR family CARD domain-containing protein 4) relates to this theme using model systems and orthogonal readouts.
- Caspases: Researchers commonly examine how NLRC4 (NLR family CARD domain-containing protein 4) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative NLRC4 (NLR family CARD domain-containing protein 4) levels across conditions; band patterns may reflect isoforms and processing.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
