| Field | Specification |
|---|---|
| Target | |
| Alternative names | Inactive caspase-12;CASP-12;CASP12;UNQ9415/PRO34398; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-12. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes inactive caspase-12 (gene CASP12) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 38.9 kDa, and the supplier reports an observed band at about 39 kDa.
Caspase-12 is a caspase family member that, in most humans, is produced as a truncated, likely inactive protein. Where the full-length form is expressed, it acts as a negative regulator of inflammation, dampening NF-κB activation and cytokine release, and it has been associated with increased susceptibility to sepsis. It is also studied in ER stress models in rodents.
| Target | Inactive caspase-12 (gene CASP12; UniProt Q6UXS9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 38.9 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): PANC cells, SMMC cells, A549 cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human mammary cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of human caspase-12.
Reactivity Notes
The supplier lists reactivity with human and rat. Tissue expression noted by UniProt (human): Widely expressed, with highest levels in lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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