| Field | Specification |
|---|---|
| Target | |
| Alternative names | Junction plakoglobin;Catenin gamma;Desmoplakin III;Desmoplakin-3;JUP;CTNNG, DP3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Catenin gamma |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOBD-10 is a rabbit monoclonal antibody that recognizes junction plakoglobin (gene JUP) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 81.7 kDa, and the supplier reports an observed band at about 82 kDa.
Plakoglobin (JUP, also called γ-catenin) is an armadillo-repeat protein closely related to β-catenin. It is a structural component of desmosomes and adherens junctions, where it links cadherins to the cytoskeleton and helps cells resist mechanical stress, and it can substitute for β-catenin in cadherin–catenin complexes. Mutations in JUP are linked to arrhythmogenic cardiomyopathy and skin disorders such as Naxos disease.
| Target | Junction plakoglobin (gene JUP; UniProt P14923, human) |
|---|---|
| Clone | AOBD-10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 81.7 kDa |
| Observed MW | 82 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human A431 cells, human HT-29 cells, human Hacat cells, human RT4 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human catenin gamma.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places junction plakoglobin in the cell junction, cytoplasm, cell membrane and nucleus. Tissue expression noted by UniProt (human): Expressed in cardiomyocytes in the heart (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
