| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cathepsin D; Ctsd |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse Cathepsin D recombinant protein (Position: K161-Q358). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Ctsd in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cathepsin D/Ctsd Antibody Picoband® catalog # A01361. Tested in ELISA, IF, ICC, and WB applications. This antibody reacts with Mouse and Rat, offering an essential tool for scientific applications such as cell lysate analysis and breast adenocarcinoma cell line research. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse Cathepsin D recombinant protein (Position: K161-Q358). (reported region: K161-Q358).
- Molecular weight context: reported MW: 45 kDa; calculated MW: nan
- Reactivity: Mouse,Rat
- Applications: ELISA, IF, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
cathepsin D. Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in signal peptide and a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation of proteins, cell invasion, and apoptosis, playing a significant role in cell signaling technology. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer's disease and it has been considered as a breast cancer tumor marker. Functional note: Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Reported localization: Lysosome. Melanosome. Secreted, extracellular space.
Research relevance and current trends
- Cancer: Researchers commonly examine how Ctsd relates to this theme using model systems and orthogonal readouts.
- Cell Adhesion Proteins: Researchers commonly examine how Ctsd relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how Ctsd relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Ctsd levels across conditions; band patterns may reflect isoforms and processing.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
