| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cathepsin G;3.4.21.20;Vimentin-specific protease;VSP;Ctsg; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse Cathepsin G recombinant protein (Position: I21-T261). Mouse Cathepsin G shares 70.2% amino acid (aa) sequence identity with human Cathepsin G. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Ctsg (Cathepsin G) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cathepsin G/CTSG Antibody Picoband® catalog # PB9855. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived mouse Cathepsin G recombinant protein (Position: I21-T261). Mouse Cathepsin G shares 70.2% amino acid (aa) sequence identity with human Cathepsin G. (reported region: I21-T261).
- Molecular weight context: reported MW: 29 kDa; calculated MW: 29096 MW
- Reactivity: Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Cathepsin G; Cathepsin G. Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSG?gene.?This gene is mapped to 14q12. In transgenic mice, it was found that?human Cathepsin G gene was expressed in early myeloid precursors in a manner coordinate with the expression of the endogenous murine gene in the bone marrow and spleen. The protein encoded by this?gene, a member of the?peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear?leukocytes.?The encoded protease has a specificity similar to that of?chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in?connective tissue?remodeling at sites of?inflammation.? Functional note: This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis. Reported localization: Membrane. Strongly associated with membranes. Expression/tissue context: Expressed in the aorta extrcellular space (at protein level). .
Research relevance and current trends
- Cytoskeleton/ECM: Researchers commonly examine how Ctsg (Cathepsin G) relates to this theme using model systems and orthogonal readouts.
- ECM Enzymes: Researchers commonly examine how Ctsg (Cathepsin G) relates to this theme using model systems and orthogonal readouts.
- Extracellular Matrix: Researchers commonly examine how Ctsg (Cathepsin G) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Ctsg (Cathepsin G) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
