| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cathepsin G;CG;3.4.21.20;CTSG; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human Cathepsin G recombinant protein (Position: R25-E252). Human Cathepsin G shares 69.7% amino acid (aa) sequence identity with mouse Cathepsin G. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CTSG (Cathepsin G) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Cathepsin G/CTSG Antibody Picoband® catalog # PB10053. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human Cathepsin G recombinant protein (Position: R25-E252). Human Cathepsin G shares 69.7% amino acid (aa) sequence identity with mouse Cathepsin G. (reported region: R25-E252).
- Molecular weight context: reported MW: 35 kDa; calculated MW: 28837 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Cathepsin G; Cathepsin G. Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSG gene. This gene is mapped to 14q12. In transgenic mice, it was found that human Cathepsin G gene was expressed in early myeloid precursors in a manner coordinate with the expression of the endogenous murine gene in the bone marrow and spleen. The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. Functional note: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. . Reported localization: Cell surface . Expression/tissue context: B-cells and in primary carcinomas.
Research relevance and current trends
- Cytoskeleton/ECM: Researchers commonly examine how CTSG (Cathepsin G) relates to this theme using model systems and orthogonal readouts.
- ECM Enzymes: Researchers commonly examine how CTSG (Cathepsin G) relates to this theme using model systems and orthogonal readouts.
- Extracellular Matrix: Researchers commonly examine how CTSG (Cathepsin G) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CTSG (Cathepsin G) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CTSG (Cathepsin G) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
