| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cathepsin S; CTSS |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cathepsin S |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFAA-3 is a rabbit monoclonal antibody that recognizes cathepsin S (gene CTSS) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 37.5 kDa, and the supplier reports an observed band at about 25 kDa.
Cathepsin S (CTSS) is a cysteine protease made mainly by antigen-presenting cells. It clears the last invariant chain fragment bound to MHC class II molecules, allowing antigenic peptides to be loaded, and it stays active at neutral pH, so it can also remodel extracellular matrix. It is studied in antigen presentation, autoimmunity, itch and inflammation.
| Target | Cathepsin S (gene CTSS; UniProt P25774, human) |
|---|---|
| Clone | AFAA-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 37.5 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): U87-MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human cathepsin S.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes cathepsin S as secreted and also located in the lysosome and cytoplasmic vesicle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
