| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-CaVpan α1 Antibody is an antibody targeting Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IF, IHC, IP, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c (also reported as Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c).
- Immunogen/epitope region: Intracellular, C-terminal region..
- Homology note: CaV1 family (α1C, α1D, α1F, α1S) from all known species - identical; CaV2 family (α1A, α1B, α1E) from all known species - 17/19 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Cited use: IP, IHC (literature use does not guarantee performance in every setup).
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
Ca2+ ions regulate many cellular processes such as secretion, proliferation, and apoptosis and they are also cofactors of many proteins.1,2 In neurons, Ca2+ entry through voltage-dependent Ca2+ (CaV) channels triggers neurotransmitter release. Fast synaptic transmission in the mammalian central nervous system (CNS) is mediated by several high voltage-dependent Ca2+ channels.3Five types of Ca2+ channels are expressed in the CNS: the L-type (CaV1), N-type (CaV2.2), P/Q-type (CaV2.1), R-type (CaV2.3), and the T-type (CaV3).4 Each CaV channel is a multimeric protein composed of a pore forming α1 subunit and the auxiliary β (CaVβ), α2δ and γ subunits. There are four known CaVβ subunits, in addition to four α2δ subunits and eight γ subunits.4
Research relevance and current trends
- Linking transporter/channel abundance to ionic homeostasis and excitability-related phenotypes.
- Studying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.
- Combining antibody readouts with functional assays for more complete interpretation.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Immunoprecipitation (IP): enrich the target for downstream detection or complex analysis (context-dependent).
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-CC004.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-CC004; Negative control: BLP-CC004.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
