| Field | Specification |
|---|---|
| Target | |
| Alternative names | Coiled-coil domain-containing protein 6;Papillary thyroid carcinoma-encoded protein;Protein H4;CCDC6;D10S170, TST1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CCDC6, identical to the related mouse sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes coiled-coil domain-containing protein 6 (gene CCDC6) in human and rat samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 53.3 kDa, and the supplier reports an observed band at about 66 kDa.
CCDC6 is a coiled-coil protein involved in several cellular processes, including regulation of phosphatases such as PP2A and PP4 and the DNA damage response. It is well known as a fusion partner of the RET kinase in the CCDC6–RET (RET/PTC1) rearrangement found in papillary thyroid carcinoma, making it relevant to cancer research.
| Target | Coiled-coil domain-containing protein 6 (gene CCDC6; UniProt Q16204, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 53.3 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat testis tissue, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): T-47D cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Synthetic peptide from the N-terminal region of human CCDC6. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places coiled-coil domain-containing protein 6 in the cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
