| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C-C motif chemokine 3;G0/G1 switch regulatory protein 19-1;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;PAT 464.1;SIS-beta;Small-inducible cytokine A3;Tonsillar lymphocyte LD78 alpha protein;MIP-1-alpha (4-69);LD78-alpha (4-69);CCL3;G0S19-1, MIP1A, SCYA3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CCL3 recombinant protein (Position: S24-A92). Human CCL3 shares 75.8% and 77.3% amino acid (aa) sequence identity with mouse and rat CCL3, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CCL3 (C-C motif chemokine 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband® catalog # PB9571. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CCL3 recombinant protein (Position: S24-A92). Human CCL3 shares 75.8% and 77.3% amino acid (aa) sequence identity with mouse and rat CCL3, respectively. (reported region: S24-A92).
- Molecular weight context: reported MW: 11 kDa; calculated MW: 10085 MW
- Reactivity: Human
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
C-C motif chemokine 3; C-C motif chemokine 3. Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for CCL3 predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. CCL3 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. CCL3 is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. Functional note: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). . Reported localization: Secreted. Expression/tissue context: Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
Research relevance and current trends
- Antiviral Signaling: Researchers commonly examine how CCL3 (C-C motif chemokine 3) relates to this theme using model systems and orthogonal readouts.
- Chemokines: Researchers commonly examine how CCL3 (C-C motif chemokine 3) relates to this theme using model systems and orthogonal readouts.
- Immune System Diseases: Researchers commonly examine how CCL3 (C-C motif chemokine 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CCL3 (C-C motif chemokine 3) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the intercrine beta (chemokine CC) family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
