| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C motif chemokine 3;G0/G1 switch regulatory protein 19-1;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;PAT 464.1;SIS-beta;Small-inducible cytokine A3;Tonsillar lymphocyte LD78 alpha protein;MIP-1-alpha (4-69);LD78-alpha (4-69);CCL3;G0S19-1, MIP1A, SCYA3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CCL3 recombinant protein (Position: S24-A92). Human CCL3 shares 75.8% and 77.3% amino acid (aa) sequence identity with mouse and rat CCL3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects C-C motif chemokine 3 (gene CCL3) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 10.1 kDa, and the supplier reports an observed band at about 11 kDa.
CCL3, also called macrophage inflammatory protein 1α (MIP-1α), is a CC chemokine released by macrophages, T cells and other immune cells during inflammation. It signals through CCR1, CCR4 and CCR5 to recruit and activate leukocytes, and because it competes with HIV-1 for CCR5, it is studied in inflammation, host defense and HIV biology.
| Target | C-C motif chemokine 3 (gene CCL3; UniProt P10147, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 10.1 kDa |
| Observed MW | 11 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| ELISA | 0.1–0.5 µg/mL |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human CCL3 fragment (Ser24–Ala92), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 75.8% and 77.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes C-C motif chemokine 3 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
