| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C motif chemokine 7; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; Small-inducible cytokine A7; Ccl7; Mcp3; Scya7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat Ccl7 recombinant protein (Position: Q24-P97). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes C-C motif chemokine 7 (gene Ccl7) in rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 10.9 kDa, and the supplier reports an observed band at about 9 kDa.
CCL7, also called monocyte chemotactic protein 3 (MCP-3), is a CC chemokine that recruits monocytes, eosinophils, basophils and T cells to sites of inflammation or infection. It binds several receptors, including CCR1, CCR2 and CCR3, and is studied alongside CCL2 in monocyte trafficking, inflammatory disease and tissue repair.
| Target | C-C motif chemokine 7 (gene Ccl7; UniProt Q9QXY8, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 10.9 kDa |
| Observed MW | 9 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL (Rat) |
Samples with a confirmed band (WB): recombinant rat Ccl7 protein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant rat Ccl7 fragment (Gln24–Pro97), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with rat. UniProt describes C-C motif chemokine 7 as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
