Anti-CCL8 Antibody

Overview

This antibody is intended for detection of Ccl8 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-CCL8 Antibody Picoband® catalog # A03237-1. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E. coli-derived mouse CCL8 recombinant protein (Position: G24-P97). (reported region: G24-P97).
• Molecular weight context: reported MW: 71 kDa; calculated MW: nan
• Reactivity: Mouse,Rat
• Applications: ELISA, IHC

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

chemokine (C-C motif) ligand 8. CCL8, also known as monocyte chemoattractant protein 2 (MCP2), is a protein that in humans is encoded by the CCL8 gene. It is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B, CCR3 and CCR5. Functional note: Chemotactic factor that attracts monocytes. This protein can bind heparin (By similarity). . Reported localization: Secreted.

Research relevance and current trends

• Chemokines: Researchers commonly examine how Ccl8 relates to this theme using model systems and orthogonal readouts.
• Immunology: Researchers commonly examine how Ccl8 relates to this theme using model systems and orthogonal readouts.
• Innate Immunity: Researchers commonly examine how Ccl8 relates to this theme using model systems and orthogonal readouts.

Common research applications

• IHC/IHC-F: assess spatial distribution of Ccl8 across tissue regions and cell types using matched controls.
• ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.