| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-C motif chemokine 8; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8; Ccl8; Mcp2; Scya8 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CCL8 recombinant protein (Position: G24-P97). |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes C-C motif chemokine 8 (gene Ccl8) in mouse and rat samples and is validated for ELISA and IHC. The predicted molecular weight is 11.0 kDa.
CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is a CC chemokine that attracts monocytes, lymphocytes, basophils and eosinophils. It binds heparin and signals through receptors such as CCR1, CCR2 and CCR5, and it is studied as a mediator of inflammatory and interferon-driven immune responses.
| Target | C-C motif chemokine 8 (gene Ccl8; UniProt Q9Z121, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 11.0 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with confirmed staining (IHC): mouse kidney tissue, mouse small intestine tissue, mouse spleen tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant mouse CCL8 fragment (Gly24–Pro97), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes C-C motif chemokine 8 as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
