| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neprilysin;3.4.24.11 ;Atriopeptidase;Common acute lymphocytic leukemia antigen;CALLA;Enkephalinase;Neutral endopeptidase 24.11;NEP;Neutral endopeptidase;Skin fibroblast elastase;SFE;CD10;MME;EPN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CD10 CD10 is a transmembrane type II molecule and functions as a metallo-peptidase requiring zinc. Specifically, CD10 cleaves 1-3 amino-terminal amino acids from peptides with a preference for neutral amino acids (valine, iso-leucine, phenylalanine, leucine or alanine) . Involved in the degradation of atrial natriuretic factor (ANF) . |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFGB-13 is a rabbit monoclonal antibody that recognizes neprilysin (gene MME) in human and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 85.5 kDa, and the supplier reports an observed band at about 100 kDa.
Neprilysin (MME, also known as CD10) is a membrane-bound zinc metalloprotease that cleaves short peptides at the cell surface. Its substrates include enkephalins, substance P, bradykinin, natriuretic peptides and amyloid-β, so it is studied in cardiovascular regulation and neurodegeneration, and CD10 is also a widely used hematopoietic cell marker.
| Target | Neprilysin (gene MME; UniProt P08473, human) |
|---|---|
| Clone | AFGB-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 85.5 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CD10 CD10 is a transmembrane type II molecule and functions as a metallo-peptidase requiring zinc.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places neprilysin in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
